6OPM
Casposase bound to integration product
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | CRISPR-associated endonuclease Cas1 | polymer | 431 | 49961.5 | 4 | UniProt (A0A0F8IEL4) Pfam (PF01867) | Methanosarcina mazei | casposase |
| 2 | E, F (E, F) | DNA 21-mer | polymer | 21 | 6383.1 | 2 | Methanosarcina mazei | ||
| 3 | G, H (H, J) | unknown | polymer | 13 | 1124.4 | 2 | Methanosarcina mazei | ||
| 4 | I, J, K, L, M... (A, C, E, F) | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 5 | O, P, Q, R, S... (A, B, C, D, E...) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 405 (UniProt: A0A0F8IEL4)
| PDB | External Database | Details |
|---|---|---|
| Gly -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Ala -14 | - | expression tag |
| Mse -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Gly -8 | - | expression tag |
| Ser -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Ser -4 | - | expression tag |
| His -3 | - | expression tag |
| Asn -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Mse 1 | - | expression tag |
| Ser 184 | Cys 184 | engineered mutation |
| Asp 406 | - | expression tag |
| Gly 407 | - | expression tag |
| Arg 408 | - | expression tag |
| Glu 409 | - | expression tag |
| Asn 410 | - | expression tag |
| Leu 411 | - | expression tag |
| Tyr 412 | - | expression tag |
| Phe 413 | - | expression tag |
| Gln 414 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 214861.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 240.5 | |
| All* | Total formula weight | 215101.5 |
