6OPM

Casposase bound to integration product

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	CRISPR-associated endonuclease Cas1	polymer	431	49961.5	4	UniProt (A0A0F8IEL4) Pfam (PF01867) In PDB	Methanosarcina mazei	casposase
2	E, F	DNA 21-mer	polymer	21	6383.1	2		Methanosarcina mazei
3	H, J	unknown	polymer	13	1124.4	2		Methanosarcina mazei
4	A, C, E, F	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
5		water	water		18.0	36	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 405 (UniProt: A0A0F8IEL4)

PDB	External Database	Details
Gly -16	-	expression tag
Ser -15	-	expression tag
Ala -14	-	expression tag
Mse -13	-	expression tag
Asp -12	-	expression tag
Pro -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Asn -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Asn -2	-	expression tag
Ser -1	-	expression tag
Leu 0	-	expression tag
Mse 1	-	expression tag
Ser 184	Cys 184	engineered mutation
Asp 406	-	expression tag
Gly 407	-	expression tag
Arg 408	-	expression tag
Glu 409	-	expression tag
Asn 410	-	expression tag
Leu 411	-	expression tag
Tyr 412	-	expression tag
Phe 413	-	expression tag
Gln 414	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	214861.0
Non-Polymers*	Number of molecules	6
	Total formula weight	240.5
All*	Total formula weight	215101.5

*Water molecules are not included.