6OMV
CryoEM structure of the LbCas12a-crRNA-AcrVA4-DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | DNA (5'-D(P*TP*AP*AP*TP*TP*TP*CP*CP*TP*AP*AP*AP*GP*GP*AP*CP*G)-3') | polymer | 17 | 5210.4 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 2 | B (F) | DNA (5'-D(*CP*GP*TP*CP*CP*TP*TP*TP*AP*GP*GP*A)-3') | polymer | 12 | 3653.4 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 3 | C, F (A, C) | AcrVA4 | polymer | 234 | 27369.2 | 2 | UniProt (A0A0U2APF4) | Moraxella bovoculi | |
| 4 | D (B) | Cpf1 | polymer | 1227 | 143750.2 | 1 | UniProt (A0A182DWE3) | Lachnospiraceae bacterium ND2006 | |
| 5 | E (G) | RNA | polymer | 40 | 12879.6 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 6 | G, H (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | I, J (A, G) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
B: 2 - 1227 (UniProt: A0A182DWE3)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Asn 112 | Ala 113 | conflict |
| Glu 113 | Ala 114 | conflict |
| Phe 131 | Ala 132 | conflict |
| Leu 132 | Ala 133 | conflict |
| Gln 264 | Ala 265 | conflict |
| Lys 269 | Ala 270 | conflict |
| Val 357 | Leu 358 | conflict |
| Arg 1076 | Ala 1077 | conflict |
| Asn 1077 | Ala 1078 | conflict |
| Pro 1078 | Ala 1079 | conflict |
| Asp 1085 | Ala 1086 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 220232.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 220280.6 |
