6OMM

Cryo-EM structure of formyl peptide receptor 2/lipoxin A4 receptor in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	N-formyl peptide receptor 2	polymer	363	40329.2	1	UniProt (P25090) Pfam (PF00001)	Homo sapiens (Human)	FMLP-related receptor I,FMLP-R-I,Formyl peptide receptor-like 1,HM63,Lipoxin A4 receptor,LXA4 receptor,RFP
2	B (L)	Peptide agonist	polymer	6	857.1	1		synthetic construct
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	353	40327.9	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39021.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7430.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
6	F (E)	scFv16	polymer	247	26323.3	1		synthetic construct
7	G, H, I, J, K (R)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)
8	L, M, N (R)	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)

Sequence modifications

R: 1 - 342 (UniProt: P25090)

PDB	External Database	Details
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ala -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

A: 2 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	engineered mutation
Ser 326	Ala 326	engineered mutation
Glu 328	Asp 328	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Glu 6	Gln 6	engineered mutation
Gln 130	Glu 130	engineered mutation
Asp 237	Asn 237	engineered mutation

C: 2 - 68 (UniProt: P59768)

PDB	External Database	Details
Gln 17	Glu 17	engineered mutation
Gln 58	Glu 58	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	154289.8
Non-Polymers*	Number of molecules	8
	Total formula weight	2702.5
All*	Total formula weight	156992.3

*Water molecules are not included.