6OMF
CryoEM structure of SigmaS-transcription initiation complex with activator Crl
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli | RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoS | polymer | 331 | 38069.8 | 1 | UniProt (S5ZIY8) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Salmonella typhimurium | Sigma S,Sigma-38 |
| 6 | G (J) | Sigma factor-binding protein Crl | polymer | 136 | 16109.3 | 1 | UniProt (A0A0F7J6B7) Pfam (PF07417) UniProt (by SIFTS) (Q7CR52) | Salmonella typhimurium | |
| 7 | H (T) | Template DNA strand | polymer | 66 | 20325.0 | 1 | Escherichia coli | ||
| 8 | I (N) | Non-template DNA strand | polymer | 66 | 20361.1 | 1 | Escherichia coli | ||
| 9 | J (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 234 (UniProt: P0A7Z6)
F: 1 - 330 (UniProt: S5ZIY8)
J: 1 - 133 (UniProt: A0A0F7J6B7)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 464220.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 464375.8 |
