6OKP

B41 SOSIP.664 in complex with the silent-face antibody SF12 and V3-targeting antibody 10-1074

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Envelope glycoprotein gp41	polymer	153	17357.8	3	UniProt (B3UEZ6) Pfam (PF00517) In PDB	Human immunodeficiency virus 1 (HIV-1)
2	D, E, F	Envelope glycoprotein gp120	polymer	516	57702.5	3	UniProt (B3UES2) Pfam (PF00516) In PDB	Human immunodeficiency virus 1 (HIV-1)
3	K	10-1074 Heavy Chain,10-1074 Heavy Chain	polymer	244	26490.6	1	UniProt (S6B291) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)
4	L	10-1074 Light Chain,10-1074 Light Chain	polymer	214	23180.8	1	UniProt (Q8N5F4) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)
5	M, O, Q	SF12 Heavy Chain,SF12 Heavy Chain	polymer	241	26045.2	3	UniProt (S6B291) Pfam (PF07686) Pfam (PF07654) In PDB	Homo sapiens (Human)
6	N, P, R	SF12 Light Chain,SF12 Light Chain	polymer	212	23237.0	3	UniProt (P01834) Pfam (PF07654) In PDB	Homo sapiens (Human)
7	G	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G80966KZ)
8	H, r, s	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1559.4	3	In PDB GlyTouCan (G52461YB)
9	i, j, I, Z	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	4	In PDB GlyTouCan (G55220VL)
10	b, d, e, f, k...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	15	In PDB GlyTouCan (G42666HT)
11	a, c, l, n, S...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
12	g	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G07617FP)
13	h	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G56014GC)
14	D, E, F, A, B...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	33	Chemie (NAG)

Sequence modifications

A, B, C: 512 - 664 (UniProt: B3UEZ6)

PDB	External Database	Details
Pro 559	Ile 563	conflict
Cys 605	Thr 609	conflict

D, E, F: 31 - 505 (UniProt: B3UES2)

PDB	External Database	Details
Met -4	-	expression tag
Asp -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Lys 0	-	expression tag
Arg 1	-	expression tag
Gly 2	-	expression tag
Leu 3	-	expression tag
Cys 4	-	expression tag
Cys 5	-	expression tag
Val 6	-	expression tag
Leu 7	-	expression tag
Leu 8	-	expression tag
Leu 9	-	expression tag
Cys 10	-	expression tag
Gly 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Phe 14	-	expression tag
Val 15	-	expression tag
Ser 16	-	expression tag
Pro 17	-	expression tag
Ser 18	-	expression tag
Gln 19	-	expression tag
Glu 20	-	expression tag
Ile 21	-	expression tag
His 22	-	expression tag
Ala 23	-	expression tag
Arg 24	-	expression tag
Phe 25	-	expression tag
Arg 26	-	expression tag
Arg 27	-	expression tag
Gly 28	-	expression tag
Ala 29	-	expression tag
Arg 30	-	expression tag
Cys 501	Ala 505	conflict

K: 1 - 114 (PDB: 6OKP)
K: 115 - 220 (UniProt: S6B291)

PDB	External Database	Details
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag

L: 6 - 103 (PDB: 6OKP)
L: 104 - 213 (UniProt: Q8N5F4)
M, O, Q: 1 - 102 (PDB: 6OKP)
M, O, Q: 103 - 220 (UniProt: S6B291)

PDB	External Database	Details
His 221	-	expression tag
His 222	-	expression tag
His 223	-	expression tag
His 224	-	expression tag

N, P, R: 1 - 104 (PDB: 6OKP)
N, P, R: 105 - 211 (UniProt: P01834)

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	422698.7
Branched	Number of molecules	31
	Total formula weight	23209.2
Non-Polymers*	Number of molecules	33
	Total formula weight	7299.9
All*	Total formula weight	453207.8

*Water molecules are not included.