6OJL

Structure of YePL2A R194K in Complex with Pentagalacturonic Acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Periplasmic pectate lyase	polymer	572	64313.8	1	UniProt (A0A0T9S209) In PDB	Yersinia enterocolitica
2	B	alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-beta-D-galactopyranuronic acid	branched		898.6	1	In PDB GlyTouCan (G93132LD)
3	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4		water	water		18.0	536	Chemie (HOH)

Sequence modifications

A: 24 - 572 (UniProt: A0A0T9S209)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Lys 194	Arg 194	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64313.8
Branched	Number of molecules	1
	Total formula weight	898.6
Non-Polymers*	Number of molecules	3
	Total formula weight	186.2
All*	Total formula weight	65398.6

*Water molecules are not included.