6OIK

Muscarinic acetylcholine receptor 2-Go complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2	polymer	353	39729.9	1	UniProt (P08172) Pfam (PF00001) In PDB	Homo sapiens (Human)
2	A	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40104.6	1	UniProt (P09471) Pfam (PF00503) In PDB	Homo sapiens (Human)
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37728.2	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	H	Antibody fragment	polymer	256	27340.5	1		Mus musculus (Mouse)
6	R	4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium	non-polymer		197.3	1	Chemie (IXO)
7	R	3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide	non-polymer		437.9	1	Chemie (2CU)

Sequence modifications

R: 3 - 359 (UniProt: P08172)

PDB	External Database	Details
Asp -4	-	expression tag
Tyr -3	-	expression tag
Lys -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Asp 1	-	expression tag
Asp 2	-	expression tag
Ala 3	Asn 3	engineered mutation
Asp 6	Asn 6	engineered mutation
Asp 9	Asn 9	engineered mutation

R: 360 - 466 (UniProt: P08172)

PDB	External Database	Details
Pro 467	-	expression tag
Ala 468	-	expression tag
Gly 469	-	expression tag
Leu 470	-	expression tag
Glu 471	-	expression tag
Val 472	-	expression tag
Leu 473	-	expression tag
Phe 474	-	expression tag
Gln 475	-	expression tag

A: 1 - 354 (UniProt: P09471)

PDB	External Database	Details
Asp 9	Glu 9	engineered mutation
Lys 10	Arg 10	engineered mutation
Val 13	Leu 13	engineered mutation
Met 18	Ala 18	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	152764.2
Non-Polymers*	Number of molecules	2
	Total formula weight	635.2
All*	Total formula weight	153399.4

*Water molecules are not included.