6OEL
Engineered Fab bound to IL-4 receptor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | engineered Interleukin-4, RGA variant | polymer | 129 | 14981.3 | 1 | UniProt (P05112) Pfam (PF00727) | Homo sapiens (Human) | IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra |
| 2 | B (B) | Interleukin-4 receptor subunit alpha | polymer | 202 | 22964.7 | 1 | UniProt (P24394) Pfam (PF09238) | Homo sapiens (Human) | IL-4RA |
| 3 | C (C) | Cytokine receptor common subunit gamma | polymer | 189 | 22816.5 | 1 | UniProt (P31785) Pfam (PF22012) Pfam (PF21605) | Homo sapiens (Human) | Interleukin-2 receptor subunit gamma, IL-2RG, gammaC, p64 |
| 4 | D (H) | engineered Fab heavy chain | polymer | 229 | 24630.6 | 1 | Homo sapiens (Human) | ||
| 5 | E (L) | engineered Fab light chain | polymer | 224 | 24394.0 | 1 | Homo sapiens (Human) | ||
| 6 | F (D) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 1 | In PDB GlyTouCan (G86851RC) | |||
| 7 | G, H, I, J, K (B, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) | |||
| 8 | L (H) | NITRATE ION | non-polymer | 62.0 | 1 | Chemie (NO3) | |||
| 9 | M, N, O, P (B, C, H, L) | water | water | 18.0 | 20 | Chemie (HOH) |
Sequence modifications
A: 1 - 129 (UniProt: P05112)
B: 2 - 199 (UniProt: P24394)
C: 39 - 227 (UniProt: P31785)
| PDB | External Database | Details |
|---|---|---|
| Arg 117 | Lys 141 | engineered mutation |
| Val 118 | Thr 142 | engineered mutation |
| Gln 121 | Arg 145 | engineered mutation |
| Ser 122 | Glu 146 | engineered mutation |
| Trp 124 | Tyr 148 | engineered mutation |
| Phe 125 | Ser 149 | engineered mutation |
| Gly 128 | Ser 152 | engineered mutation |
| Ala 129 | Ser 153 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Phe 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 53 | Asn 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 109787.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 367.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1168.0 | |
| All* | Total formula weight | 111322.5 |
