6O96

Dot1L bound to the H2BK120 Ubiquitinated nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	123	13629.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (146-MER)	polymer	146	44824.6	1		synthetic construct
6	J (J)	DNA (146-MER)	polymer	146	45304.9	1		synthetic construct
7	K (K)	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	331	38245.4	1	UniProt (Q8TEK3) Pfam (PF08123)	Homo sapiens (Human)	DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4
8	L (L)	Polyubiquitin-B	polymer	76	8622.9	1	UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47)	Homo sapiens (Human)
9	M (K)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Met 3	-	expression tag
Thr 32	Ser 33	engineered mutation
Cys 120	Lys 121	engineered mutation

L: 1 - 76 (UniProt: J3QS39)

PDB	External Database	Details
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	245873.1
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	246257.6

*Water molecules are not included.