6O7I
Cryo-EM structure of Csm-crRNA-target RNA ternary bigger complex in complex with cA4 in type III-A CRISPR-Cas system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 791 | 89706.6 | 1 | UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) In PDB | Thermococcus onnurineus (strain NA1) | ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1 |
| 2 | B, J | Csm2 | polymer | 187 | 21210.3 | 2 | UniProt (B6YWB9) Pfam (PF03750) In PDB | Thermococcus onnurineus (strain NA1) | |
| 3 | C, D, K | Csm3 | polymer | 291 | 32809.0 | 3 | UniProt (B6YWC0) Pfam (PF03787) In PDB | Thermococcus onnurineus (strain NA1) | |
| 4 | E | Csm4 | polymer | 289 | 32345.1 | 1 | UniProt (B6YWC1) In PDB | Thermococcus onnurineus (strain NA1) | |
| 5 | G | RNA (38-MER) | polymer | 38 | 12463.4 | 1 | Thermococcus onnurineus | ||
| 6 | H | RNA (40-MER) | polymer | 40 | 12750.7 | 1 | Thermococcus onnurineus | ||
| 7 | I | RNA (5'-R(P*AP*AP*AP*A)-3') | polymer | 4 | 1271.9 | 1 | synthetic construct | ||
| 8 | F | Csm5 | polymer | 378 | 40588.4 | 1 | Pfam (PF03787) UniProt (by SIFTS) (B6YWC2) In PDB | Thermococcus onnurineus (strain NA1) | |
| 9 | A, C, D, K | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A: 1 - 777 (UniProt: B6YWB8)
B, J: 0 - 186 (UniProt: B6YWB9)
C, D, K: 1 - 290 (UniProt: B6YWC0)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Ala 589 | Asp 589 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 329973.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 261.6 | |
| All* | Total formula weight | 330235.3 |
