6O7I

Cryo-EM structure of Csm-crRNA-target RNA ternary bigger complex in complex with cA4 in type III-A CRISPR-Cas system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	791	89706.6	1	UniProt (B6YWB8)	Thermococcus onnurineus (strain NA1)	ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1
2	B, F (B, J)	Csm2	polymer	187	21210.3	2	UniProt (B6YWB9)	Thermococcus onnurineus (strain NA1)
3	C, D, I (C, D, K)	Csm3	polymer	291	32809.0	3	UniProt (B6YWC0)	Thermococcus onnurineus (strain NA1)
4	E (E)	Csm4	polymer	289	32345.1	1	UniProt (B6YWC1)	Thermococcus onnurineus (strain NA1)
5	G (G)	RNA (38-MER)	polymer	38	12463.4	1		Thermococcus onnurineus
6	H (H)	RNA (40-MER)	polymer	40	12750.7	1		Thermococcus onnurineus
7	J (I)	RNA (5'-R(P*AP*AP*AP*A)-3')	polymer	4	1271.9	1		synthetic construct
8	K (F)	Csm5	polymer	378	40588.4	1	UniProt (by SIFTS) (B6YWC2)	Thermococcus onnurineus (strain NA1)
9	L, M, N, O (A, C, D, K)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 1 - 777 (UniProt: B6YWB8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 589	Asp 589	engineered mutation

B, J: 0 - 186 (UniProt: B6YWB9)

PDB	External Database	Details
Ser -1	-	expression tag

C, D, K: 1 - 290 (UniProt: B6YWC0)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	329973.6
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	330235.3

*Water molecules are not included.