6O7B
Crystal structure of Csm1-Csm4 cassette in complex with cA4
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) | polymer | 791 | 89706.6 | 1 | UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335) | Thermococcus onnurineus (strain NA1) | ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1 |
| 2 | B (B) | Csm4 | polymer | 289 | 32345.1 | 1 | UniProt (B6YWC1) | Thermococcus onnurineus | |
| 3 | C, D (C, D) | Cyclic RNA cA4 | polymer | 4 | 1271.9 | 2 | synthetic construct | ||
| 4 | E, F (A, B) | water | water | 18.0 | 28 | Chemie (HOH) |
Sequence modifications
A: 1 - 777 (UniProt: B6YWB8)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Ala 589 | Asp 589 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 124595.4 | |
| All* | Total formula weight | 124595.4 |
