6O79

Crystal structure of Csm1-Csm4 cassette in complex with cA3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)	polymer	791	89706.6	1	UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) In PDB	Thermococcus onnurineus	ssDNase Cas10,Cyclic oligoadenylate synthase,ToCsm1
2	B	Csm4	polymer	289	32345.1	1	UniProt (B6YWC1) In PDB	Thermococcus onnurineus (strain NA1)
3	C	cyclic RNA cA3	polymer	3	942.7	1		synthetic construct	RNA (5'-R(P*AP*AP*A)-3')

Sequence modifications

A: 1 - 777 (UniProt: B6YWB8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Ala 589	Asp 589	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	122994.3
All*	Total formula weight	122994.3

*Water molecules are not included.