6O70

Crystal structure of Csm6 H132A mutant in complex with cA4 by cocrystallization of cA4 and Csm6 H132A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Csm6	polymer	440	49728.0	2	UniProt (B6YWC3) Pfam (PF22230)	Thermococcus onnurineus (strain NA1)
2	C, E (A, B)	2',3'- cyclic AMP	non-polymer		329.2	2	Chemie (ACK)
3	D, F (A, B)	3'-O-[(R)-{[(2S,3aS,4S,6S,6aS)-6-(6-amino-9H-purin-9-yl)-2-hydroxy-2-oxotetrahydro-2H-2lambda~5~-furo[3,4-d][1,3,2]dioxaphosphol-4-yl]methoxy}(hydroxy)phosphoryl]adenosine	non-polymer		658.4	2	Chemie (LQJ)
4	G, H (A, B)	water	water		18.0	45	Chemie (HOH)

Sequence modifications

A, B: 1 - 432 (UniProt: B6YWC3)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ala 132	His 132	engineered mutation
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99456.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1975.2
All*	Total formula weight	101431.3

*Water molecules are not included.