6O44
Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Nattokinase | polymer | 284 | 28862.8 | 2 | UniProt (A0A024B5N4) Pfam (PF00082) UniProt (by SIFTS) (P04189) | Bacillus subtilis | |
| 2 | C, D, J, K (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 3 | E (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 4 | F, G, H, I, L... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 5 | N, O (A, B) | water | water | 18.0 | 452 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 275 (UniProt: A0A024B5N4)
| PDB | External Database | Details |
|---|---|---|
| Cys 221 | Ser 327 | engineered mutation |
| Ser 276 | - | expression tag |
| Leu 277 | - | expression tag |
| Glu 278 | - | expression tag |
| His 279 | - | expression tag |
| His 280 | - | expression tag |
| His 281 | - | expression tag |
| His 282 | - | expression tag |
| His 283 | - | expression tag |
| His 284 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 57725.6 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 628.8 | |
| All* | Total formula weight | 58354.4 |
