6O0Y
Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1372 | 158986.1 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | single guide RNA | polymer | 102 | 33059.7 | 1 | synthetic construct | ||
| 3 | C (C) | 5' product of target strand DNA | polymer | 13 | 3936.6 | 1 | synthetic construct | ||
| 4 | D (D) | non-target strand DNA | polymer | 40 | 12452.0 | 1 | synthetic construct | ||
| 5 | E (c) | 3' product of target strand DNA | polymer | 27 | 8194.3 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 216628.7 | |
| All* | Total formula weight | 216628.7 |
