6NZO

Set2 bound to nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3	polymer	136	15437.1	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P02302) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Ubiquitin-60S ribosomal protein L40,Histone H2A	polymer	221	24045.4	2	UniProt (P0CH09) UniProt (Q6AZJ8) Pfam (PF00240) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	D, H	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	DNA (149-MER)	polymer	149	45764.1	1		synthetic construct
6	J	DNA (149-MER)	polymer	149	46222.4	1		synthetic construct
7	S	Histone-lysine N-methyltransferase	polymer	939	105425.9	1	UniProt (G0S676) Pfam (PF00397) Pfam (PF08236) Pfam (PF17907) Pfam (PF00856) Pfam (PF08711) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
8	S	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A, E: 0 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Met 36	Lys 37	engineered mutation

C, G: -71 - 3 (UniProt: P0CH09)

PDB	External Database	Details
Met -91	-	initiating methionine
Asp -90	-	expression tag
Ser -89	-	expression tag
Pro -88	-	expression tag
Asp -87	-	expression tag
Leu -86	-	expression tag
His -85	-	expression tag
His -84	-	expression tag
His -83	-	expression tag
His -82	-	expression tag
His -81	-	expression tag
His -80	-	expression tag
Gly -79	-	expression tag
Thr -78	-	expression tag
Leu -77	-	expression tag
Val -76	-	expression tag
Pro -75	-	expression tag
Arg -74	-	expression tag
Gly -73	-	expression tag
Ser -72	-	expression tag
Thr 4	-	linker
Ser 5	-	linker
Ser 6	-	linker
Gly 7	-	linker
Gly 8	-	linker
Ser 9	-	linker
Gly 10	-	linker
Gly 11	-	linker
Ser 12	-	linker
Gly 13	-	linker
Gly 14	-	linker
Ser 15	-	linker
Gly 16	-	linker

C, G: 17 - 129 (UniProt: Q6AZJ8)
D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	326478.4
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	326674.6

*Water molecules are not included.