6NUU
Structure of Calcineurin mutant in complex with NHE1 peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | polymer | 372 | 42695.9 | 1 | UniProt (Q08209) Pfam (PF00149) | Homo sapiens (Human) | CAM-PRP catalytic subunit,Calmodulin-dependent calcineurin A subunit alpha isoform |
| 2 | B (B) | Calcineurin subunit B type 1 | polymer | 156 | 17755.2 | 1 | UniProt (P63098) Pfam (PF13499) | Homo sapiens (Human) | Protein phosphatase 2B regulatory subunit 1,Protein phosphatase 3 regulatory subunit B alpha isoform 1 |
| 3 | C (C) | Sodium/hydrogen exchanger 1 | polymer | 48 | 5268.0 | 1 | UniProt (P19634) | Homo sapiens (Human) | APNH,Na(+)/H(+) antiporter,amiloride-sensitive,Na(+)/H(+) exchanger 1,NHE-1,Solute carrier family 9 member 1 |
| 4 | D (A) | FE (III) ION | non-polymer | 55.8 | 1 | Chemie (FE) | |||
| 5 | E (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | F (A) | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
| 7 | G (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 8 | H, I, J, K (B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 9 | L, M, N (A, B, C) | water | water | 18.0 | 256 | Chemie (HOH) |
Sequence modifications
A: 1 - 370 (UniProt: Q08209)
B: 16 - 170 (UniProt: P63098)
C: 679 - 723 (UniProt: P19634)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 155 | His 155 | engineered mutation |
| Ala 159 | Tyr 159 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 15 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 676 | - | expression tag |
| His 677 | - | expression tag |
| Met 678 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 65719.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 482.7 | |
| All* | Total formula weight | 66201.7 |
