6NPY

Cryo-EM structure of NLRP3 bound to NEK7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NACHT, LRR and PYD domains-containing protein 3	polymer	1034	117891.0	1	UniProt (Q96P20) Pfam (PF02758) Pfam (PF14484) Pfam (PF05729) Pfam (PF17779) Pfam (PF17776) Pfam (PF13516) In PDB	Homo sapiens (Human)	Angiotensin/vasopressin receptor AII/AVP-like,Caterpiller protein 1.1,CLR1.1,Cold-induced autoinflammatory syndrome 1 protein,Cryopyrin,PYRIN-containing APAF1-like protein 1
2	B	Protein kinase R,Serine/threonine-protein kinase Nek7	polymer	279	32015.2	1	UniProt (P19525) UniProt (Q8TDX7) Pfam (PF00069) In PDB	Homo sapiens (Human)	Eukaryotic translation initiation factor 2-alpha kinase 2,eIF-2A protein kinase 2,Interferon-inducible RNA-dependent protein kinase,P1/eIF-2A protein kinase,Protein kinase RNA-activated,Protein kinase R,Tyrosine-protein kinase EIF2AK2,p68 kinase,Never in mitosis A-related kinase 7,NimA-related protein kinase 7
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

A: 1 - 1034 (UniProt: Q96P20)

PDB	External Database	Details
Ile 133	Asp 135	engineered mutation
Cys 135	Arg 137	engineered mutation
Ala 136	Lys 138	engineered mutation
Ala 140	Lys 142	engineered mutation

B: 27 - 33 (UniProt: P19525)

PDB	External Database	Details
Gly 24	-	expression tag
Ser 25	-	expression tag
Thr 26	-	expression tag

B: 34 - 302 (UniProt: Q8TDX7)

PDB	External Database	Details
Arg 54	Leu 54	engineered mutation
Lys 58	Val 58	engineered mutation
Thr 59	Pro 59	engineered mutation
Ala 87	Lys 87	engineered mutation
Val 99	Ala 99	engineered mutation
Cys 100	Ser 100	engineered mutation
Thr 103	Glu 103	engineered mutation
Gly 104	Asp 104	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	149906.1
Non-Polymers*	Number of molecules	1
	Total formula weight	427.2
All*	Total formula weight	150333.3

*Water molecules are not included.