6NMD

cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cpf1	polymer	1227	143750.2	1	UniProt (A0A182DWE3) Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) In PDB	Lachnospiraceae bacterium ND2006
2	G	crRNA	polymer	40	12879.6	1		Lachnospiraceae bacterium ND2006
3	B	AcrVA1	polymer	174	20682.5	1	UniProt (A0A0U2BNN7) UniProt (by SIFTS) (A0A5H1ZR47) In PDB	Moraxella bovoculi
4	A, G	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A: 2 - 1227 (UniProt: A0A182DWE3)

PDB	External Database	Details
Met 1	-	expression tag
Asn 112	Ala 113	conflict
Glu 113	Ala 114	conflict
Phe 131	Ala 132	conflict
Leu 132	Ala 133	conflict
Gln 264	Ala 265	conflict
Lys 269	Ala 270	conflict
Val 357	Leu 358	conflict
Arg 1076	Ala 1077	conflict
Asn 1077	Ala 1078	conflict
Pro 1078	Ala 1079	conflict
Asp 1085	Ala 1086	conflict

B: 5 - 174 (UniProt: A0A0U2BNN7)

PDB	External Database	Details
Met 1	-	expression tag
Ser 2	-	expression tag
Lys 3	-	expression tag
Ala 4	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	177312.4
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	177361.0

*Water molecules are not included.