6NMD
cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cpf1 | polymer | 1227 | 143750.2 | 1 | UniProt (A0A182DWE3) | Lachnospiraceae bacterium ND2006 | |
| 2 | B (G) | crRNA | polymer | 40 | 12879.6 | 1 | Lachnospiraceae bacterium ND2006 | ||
| 3 | C (B) | AcrVA1 | polymer | 174 | 20682.5 | 1 | UniProt (A0A0U2BNN7) UniProt (by SIFTS) (A0A5H1ZR47) | Moraxella bovoculi | |
| 4 | D, E (A, G) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F, G (A, G) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A: 2 - 1227 (UniProt: A0A182DWE3)
B: 5 - 174 (UniProt: A0A0U2BNN7)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Asn 112 | Ala 113 | conflict |
| Glu 113 | Ala 114 | conflict |
| Phe 131 | Ala 132 | conflict |
| Leu 132 | Ala 133 | conflict |
| Gln 264 | Ala 265 | conflict |
| Lys 269 | Ala 270 | conflict |
| Val 357 | Leu 358 | conflict |
| Arg 1076 | Ala 1077 | conflict |
| Asn 1077 | Ala 1078 | conflict |
| Pro 1078 | Ala 1079 | conflict |
| Asp 1085 | Ala 1086 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Ser 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Ala 4 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 177312.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 177361.0 |
