6NM9
CryoEM structure of the LbCas12a-crRNA-AcrVA4 dimer
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | AcrVA4 | polymer | 234 | 27369.2 | 2 | UniProt (A0A0U2APF4) In PDB | Moraxella bovoculi | |
2 | B, D | Cpf1 | polymer | 1227 | 143750.2 | 2 | UniProt (A0A182DWE3) Pfam (PF18501) Pfam (PF18516) Pfam (PF18510) In PDB | Lachnospiraceae bacterium ND2006 | |
3 | G, E | RNA (25-MER) | polymer | 40 | 12879.6 | 2 | Lachnospiraceae bacterium ND2006 | ||
4 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
B, D: 2 - 1227 (UniProt: A0A182DWE3)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Asn 112 | Ala 113 | conflict |
Glu 113 | Ala 114 | conflict |
Phe 131 | Ala 132 | conflict |
Leu 132 | Ala 133 | conflict |
Gln 264 | Ala 265 | conflict |
Lys 269 | Ala 270 | conflict |
Val 357 | Leu 358 | conflict |
Arg 1076 | Ala 1077 | conflict |
Asn 1077 | Ala 1078 | conflict |
Pro 1078 | Ala 1079 | conflict |
Asp 1085 | Ala 1086 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 367998.0 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 97.2 | |
All* | Total formula weight | 368095.3 |