6NL9

Crystal structure of de novo designed metal-controlled dimer of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (L12H, T16L, V29H, Y33H, N37L)-apo

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Immunoglobulin G-binding protein G	polymer	56	6251.9	4	UniProt (P19909) Pfam (PF01378) In PDB	Streptococcus	IgG-binding protein G
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	D	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
4		water	water		18.0	143	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 56 (UniProt: P19909)

PDB	External Database	Details
Met 1	-	initiating methionine
His 12	Leu 313	engineered mutation
Leu 16	Thr 317	engineered mutation
His 29	Val 330	engineered mutation
His 33	Tyr 334	engineered mutation
Leu 37	Asn 338	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	25007.5
Non-Polymers*	Number of molecules	3
	Total formula weight	70.3
All*	Total formula weight	25077.8

*Water molecules are not included.