6NL6

Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21E, T25L, K28Y, V29I, K31R, Q32H, Y33L, N35K, D36H, N37Q)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Immunoglobulin G-binding protein G	polymer	56	6348.1	4	UniProt (P19909) Pfam (PF01378) In PDB	Streptococcus	IgG-binding protein G
2	A, B, C, D	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
3	B, C, D	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
4		water	water		18.0	264	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 56 (UniProt: P19909)

PDB	External Database	Details
Met 1	-	initiating methionine
Phe 16	Thr 317	engineered mutation
Ala 18	Thr 319	engineered mutation
Glu 21	Val 322	engineered mutation
Leu 25	Thr 326	engineered mutation
Tyr 28	Lys 329	engineered mutation
Ile 29	Val 330	engineered mutation
Arg 31	Lys 332	engineered mutation
His 32	Gln 333	engineered mutation
Leu 33	Tyr 334	engineered mutation
Lys 35	Asn 336	engineered mutation
His 36	Asp 337	engineered mutation
Gln 37	Asn 338	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	25392.3
Non-Polymers*	Number of molecules	10
	Total formula weight	534.3
All*	Total formula weight	25926.5

*Water molecules are not included.