6NKY
Ternary complex crystal structure of K289M variant of DNA polymerase Beta with "hot-spot sequence" with beta-gamma CHF analogue of dGTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | DNA (5'-D(*CP*CP*GP*AP*AP*CP*AP*AP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 16 | 4861.2 | 1 | Homo sapiens | ||
| 2 | B (P) | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*TP*(2DT))-3') | polymer | 10 | 3035.0 | 1 | Homo sapiens | ||
| 3 | C (D) | DNA (5'-D(P*TP*TP*CP*GP*G)-3') | polymer | 5 | 1511.0 | 1 | Homo sapiens | ||
| 4 | D (A) | DNA polymerase beta | polymer | 335 | 38243.7 | 1 | UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | |
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F, G, H (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 7 | I, J, K (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 8 | L (A) | 2'-DEOXY-5'-O-[(R)-{[(R)-[(R)-FLUORO(PHOSPHONO)METHYL](HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]GUANOSINE | non-polymer | 523.2 | 1 | Chemie (GFH) | |||
| 9 | M, N, O, P (T, P, D, A) | water | water | 18.0 | 234 | Chemie (HOH) |
Sequence modifications
A: 1 - 335 (UniProt: P06746)
| PDB | External Database | Details |
|---|---|---|
| Met 289 | Lys 289 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 47650.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 722.8 | |
| All* | Total formula weight | 48373.7 |
