6NJG

Ubiquitin Variant in Complex with Ubiquitin Interacting Motif

Replaces:  5UCL

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Vacuolar protein sorting-associated protein 27	polymer	24	2824.1	1	UniProt (P40343) Pfam (PF02809)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Golgi retention defective protein 11
2	B (C)	Polyubiquitin-B	polymer	89	9742.1	1	UniProt (J3QS39) Pfam (PF00240)	Homo sapiens (Human)
3	C, D (B, C)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

B: 1 - 23 (UniProt: P40343)

PDB	External Database	Details
Tyr 0	-	expression tag

C: 1 - 78 (UniProt: J3QS39)

PDB	External Database	Details
Gly -6	-	expression tag
Gly -5	-	expression tag
Ala -4	-	expression tag
Ala -3	-	expression tag
Gln -2	-	expression tag
Pro -1	-	expression tag
Ala 0	-	expression tag
Gln 6	Lys 6	engineered mutation
Ile 8	Leu 8	engineered mutation
Val 10	Gly 10	engineered mutation
Met 11	Lys 11	engineered mutation
Arg 12	Thr 12	engineered mutation
Ala 14	Thr 14	engineered mutation
Met 48	Lys 48	engineered mutation
Lys 62	Gln 62	engineered mutation
Arg 63	Lys 63	engineered mutation
Asp 64	Glu 64	engineered mutation
Asn 66	Thr 66	engineered mutation
Tyr 68	His 68	engineered mutation
Ser 71	Leu 71	engineered mutation
Ser 72	Arg 72	engineered mutation
Ser 75	Gly 75	engineered mutation
Leu 76	Gly 76	engineered mutation
Arg 77	Met 77	engineered mutation
Ala 78	Gln 78	engineered mutation
Gly 79	-	expression tag
Ala 80	-	expression tag
Ala 81	-	expression tag
Ala 82	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	12566.2
All*	Total formula weight	12566.2

*Water molecules are not included.