6NJ6
Thermostable variant of human carbonic anhydrase with tetrazine 2.0 at site 186 reacted with sTCO in crystallo
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Carbonic anhydrase 2 | polymer | 266 | 30241.9 | 2 | UniProt (P00918) Pfam (PF00194) In PDB | Homo sapiens (Human) | Carbonate dehydratase II,Carbonic anhydrase C,CAC,Carbonic anhydrase II,CA-II |
2 | A, B | METHANOL | non-polymer | 32.0 | 2 | Chemie (MOH) | |||
3 | A, B | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
5 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
6 | water | water | 18.0 | 630 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 260 (UniProt: P00918)
PDB | External Database | Details |
---|---|---|
Met 0 | - | initiating methionine |
Ala 1 | - | expression tag |
Thr 64 | Ala 65 | engineered mutation |
His 99 | Leu 100 | engineered mutation |
Asn 153 | Lys 153 | engineered mutation |
Djd 186 | Glu 186 | engineered mutation |
Ser 223 | Leu 223 | engineered mutation |
Pro 239 | Leu 239 | engineered mutation |
Thr 247 | Ala 247 | engineered mutation |
His 261 | - | expression tag |
His 262 | - | expression tag |
His 263 | - | expression tag |
His 264 | - | expression tag |
His 265 | - | expression tag |
His 266 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 60483.8 | |
Non-Polymers* | Number of molecules | 14 |
Total formula weight | 1135.7 | |
All* | Total formula weight | 61619.5 |