6ND1

CryoEM structure of the Sec Complex from yeast

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Protein transport protein SEC61	polymer	480	52978.1	1	UniProt (P32915) Pfam (PF10559) Pfam (PF00344) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sec61 complex subunit SEC61,Sec61 complex subunit alpha
2	A	Protein translocation protein SEC63	polymer	677	76831.6	1	UniProt (P14906) Pfam (PF00226) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Protein NPL1,Sec62/63 complex 73 kDa subunit
3	C	Protein transport protein SSS1	polymer	80	8958.6	1	UniProt (P35179) Pfam (PF00584) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sec61 complex subunit SSS1,Sec61 complex subunit gamma,Ssh1 complex subunit SSS1,Ssh1 complex subunit gamma
4	D	Protein transport protein SBH1	polymer	82	8723.2	1	UniProt (P52870) Pfam (PF03911) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sec61 complex subunit SBH1,Sec61 complex subunit beta
5	E	Translocation protein SEC66	polymer	206	24263.9	1	UniProt (P33754) Pfam (PF09802) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Protein HSS1,Sec62/63 complex 31.5 kDa subunit
6	F	Translocation protein SEC72	polymer	193	21631.1	1	UniProt (P39742) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sec62/63 complex 23 kDa subunit,p23

Sequence modifications

A: 1 - 663 (UniProt: P14906)

PDB	External Database	Details
Gly 664	-	expression tag
Ser 665	-	expression tag
Gly 666	-	expression tag
Gly 667	-	expression tag
Ser 668	-	expression tag
Gly 669	-	expression tag
Asp 670	-	expression tag
Tyr 671	-	expression tag
Lys 672	-	expression tag
Asp 673	-	expression tag
Asp 674	-	expression tag
Asp 675	-	expression tag
Asp 676	-	expression tag
Lys 677	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	193386.6
All*	Total formula weight	193386.6

*Water molecules are not included.