6NBI
Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Parathyroid hormone/parathyroid hormone-related peptide receptor | polymer | 478 | 54755.0 | 1 | UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) In PDB | Homo sapiens (Human) | PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor |
2 | P | Long-acting parathyroid hormone analog | polymer | 36 | 4274.0 | 1 | Homo sapiens | ||
3 | A | Gs protein alpha subunit | polymer | 378 | 43867.8 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63097) UniProt (by SIFTS) (P04896) In PDB | Bos taurus | |
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF00400) In PDB | Rattus norvegicus (Rat) | Transducin beta chain 1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (Bovine) | G gamma-I |
6 | N | Nanobody-35 | polymer | 126 | 13711.3 | 1 | synthetic construct | ||
7 | R | CHOLESTEROL | non-polymer | 386.7 | 3 | Chemie (CLR) |
Sequence modifications
R: 27 - 502 (UniProt: Q03431)
B: 2 - 340 (UniProt: P54311)
PDB | External Database | Details |
---|---|---|
Ala 188 | Gly 188 | engineered mutation |
Glu 503 | - | expression tag |
Phe 504 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 162384.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 1160.0 | |
All* | Total formula weight | 163544.7 |