6N9X
Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS3)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | DNA primase/helicase | polymer | 566 | 62734.4 | 6 | UniProt (P03692) Pfam (PF08273) Pfam (PF21268) Pfam (PF01751) Pfam (PF03796) In PDB | Enterobacteria phage T7 | Gene product 4,Gp4 |
2 | H | DNA-directed DNA polymerase | polymer | 704 | 79703.6 | 1 | UniProt (P00581) Pfam (PF00476) In PDB | Enterobacteria phage T7 | Gene product 5,Gp5 |
3 | P | Primer | polymer | 6 | 1842.2 | 1 | Enterobacteria phage T7 | ||
4 | T | Template | polymer | 44 | 13402.6 | 1 | Enterobacteria phage T7 | ||
5 | B | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
6 | B, C, D, E, H | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 5 | Chemie (TTP) | |||
7 | B, C, D, E, H | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) |
Sequence modifications
A, B, C, D, E, F: 1 - 566 (UniProt: P03692)
H: 1 - 704 (UniProt: P00581)
PDB | External Database | Details |
---|---|---|
Gln 343 | Glu 343 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ala 5 | Asp 5 | engineered mutation |
Ala 7 | Glu 7 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 9 |
Total formula weight | 471354.9 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 2622.1 | |
All* | Total formula weight | 473977.0 |