6N9V

Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	DNA primase/helicase	polymer	566	62734.4	6	UniProt (P03692) Pfam (PF08273) Pfam (PF21268) Pfam (PF01751) Pfam (PF03796) In PDB	Enterobacteria phage T7	Gene product 4,Gp4
2	H	DNA-directed DNA polymerase	polymer	704	79703.6	1	UniProt (P00581) Pfam (PF00476) In PDB	Enterobacteria phage T7 (Bacteriophage T7)	Gene product 5,Gp5
3	P	Primer	polymer	6	1842.2	1		Enterobacteria phage T7
4	T	Template	polymer	44	13402.6	1		Enterobacteria phage T7
5	B, C, D, E, F...	THYMIDINE-5'-TRIPHOSPHATE	non-polymer		482.2	6	Chemie (TTP)
6	B, C, D, E, F...	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
7	E	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B, C, D, E, F: 1 - 566 (UniProt: P03692)

PDB	External Database	Details
Gln 343	Glu 343	engineered mutation

H: 1 - 704 (UniProt: P00581)

PDB	External Database	Details
Ala 5	Asp 5	engineered mutation
Ala 7	Glu 7	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	471354.9
Non-Polymers*	Number of molecules	14
	Total formula weight	3128.6
All*	Total formula weight	474483.5

*Water molecules are not included.