6N9V
Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | DNA primase/helicase | polymer | 566 | 62734.4 | 6 | UniProt (P03692) Pfam (PF08273) Pfam (PF21268) Pfam (PF01751) Pfam (PF03796) | Enterobacteria phage T7 | Gene product 4,Gp4 |
| 2 | G (H) | DNA-directed DNA polymerase | polymer | 704 | 79703.6 | 1 | UniProt (P00581) Pfam (PF00476) | Enterobacteria phage T7 (Bacteriophage T7) | Gene product 5,Gp5 |
| 3 | H (P) | Primer | polymer | 6 | 1842.2 | 1 | Enterobacteria phage T7 | ||
| 4 | I (T) | Template | polymer | 44 | 13402.6 | 1 | Enterobacteria phage T7 | ||
| 5 | J, L, N, Q, S... (B, C, D, E, F...) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 6 | Chemie (TTP) | |||
| 6 | K, M, O, R, T... (B, C, D, E, F...) | MAGNESIUM ION | non-polymer | 24.3 | 7 | Chemie (MG) | |||
| 7 | P (E) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A, B, C, D, E, F: 1 - 566 (UniProt: P03692)
H: 1 - 704 (UniProt: P00581)
| PDB | External Database | Details |
|---|---|---|
| Gln 343 | Glu 343 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 5 | Asp 5 | engineered mutation |
| Ala 7 | Glu 7 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 471354.9 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 3128.6 | |
| All* | Total formula weight | 474483.5 |
