6N7P

S. cerevisiae spliceosomal E complex (UBC4)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	U1 small nuclear ribonucleoprotein 70 kDa homolog	polymer	300	34506.1	1	UniProt (Q00916) Pfam (PF12220) Pfam (PF00076)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1
2	B (B)	U1 small nuclear ribonucleoprotein C	polymer	231	27106.0	1	UniProt (Q05900) Pfam (PF06220)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U1C
3	C (C)	U1 small nuclear ribonucleoprotein A	polymer	350	40414.0	1	UniProt (P32605) Pfam (PF00076)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U1A,Mutant U1 die protein 1
4	D (D)	U1 small nuclear ribonucleoprotein component PRP42	polymer	544	65222.0	1	UniProt (Q03776)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	U1 snRNP protein PRP42,65 kDa snRNP protein,Pre-mRNA-processing factor 42
5	E (E)	Pre-mRNA-processing factor 39	polymer	629	74834.7	1	UniProt (P39682)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
6	F (F)	Protein NAM8	polymer	523	57010.8	1	UniProt (Q00539) Pfam (PF00076)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
7	G (G)	56 kDa U1 small nuclear ribonucleoprotein component	polymer	492	56575.3	1	UniProt (Q03782) Pfam (PF19097)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
8	H (H)	U1 small nuclear ribonucleoprotein component SNU71,U1 small nuclear ribonucleoprotein component SNU71,Snu71	polymer	105	10485.3	1	UniProt (P53207)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
9	I (I)	Protein LUC7	polymer	261	30245.9	1	UniProt (Q07508) Pfam (PF03194)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
10	J (J)	Pre-mRNA-processing protein PRP40	polymer	583	69176.0	1	UniProt (P33203) Pfam (PF00397) Pfam (PF01846)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
11	K (K)	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	1	UniProt (P40018) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-B,Sm protein B,SmB
12	L (L)	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	1	UniProt (Q02260) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D1,snRNP core protein D1
13	M (M)	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	1	UniProt (Q06217) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D2,snRNP core protein D2
14	N (N)	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	1	UniProt (P43321) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Sm-D3,snRNP core protein D3
15	O (O)	Small nuclear ribonucleoprotein E	polymer	94	10385.1	1	UniProt (Q12330) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-E,Sm protein E,SmE
16	P (P)	Small nuclear ribonucleoprotein F	polymer	86	9669.9	1	UniProt (P54999) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-F,Sm protein F,SmF
17	Q (Q)	Small nuclear ribonucleoprotein G	polymer	77	8490.8	1	UniProt (P40204) Pfam (PF01423)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	snRNP-G,Sm protein G,SmG
18	R (R)	U1 snRNA	polymer	568	182114.5	1	GenBank (1039023756)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
19	S (r)	UBC4 pre-mRNA	polymer	253	81030.8	1		Saccharomyces cerevisiae S288C
20	T (X)	Nuclear cap-binding protein complex subunit 1	polymer	861	100115.0	1	UniProt (P34160) Pfam (PF02854) Pfam (PF09088) Pfam (PF09090)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	80 kDa nuclear cap-binding protein,NCBP 80 kDa subunit,Glycolysis regulation protein 3,Protein SUT1,Suppressor of TOP1 protein
21	U (Y)	Nuclear cap-binding protein subunit 2	polymer	208	23803.5	1	UniProt (Q08920) Pfam (PF00076)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	20 kDa nuclear cap-binding protein,NCBP 20 kDa subunit,CBP20
22	V, W, X (B, I)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

C: 1 - 298 (UniProt: P32605)

PDB	External Database	Details
Gly 299	-	expression tag
Arg 300	-	expression tag
Arg 301	-	expression tag
Ile 302	-	expression tag
Pro 303	-	expression tag
Gly 304	-	expression tag
Leu 305	-	expression tag
Ile 306	-	expression tag
Asn 307	-	expression tag
Pro 308	-	expression tag
Trp 309	-	expression tag
Lys 310	-	expression tag
Arg 311	-	expression tag
Arg 312	-	expression tag
Trp 313	-	expression tag
Lys 314	-	expression tag
Lys 315	-	expression tag
Asn 316	-	expression tag
Phe 317	-	expression tag
Ile 318	-	expression tag
Ala 319	-	expression tag
Val 320	-	expression tag
Ser 321	-	expression tag
Ala 322	-	expression tag
Ala 323	-	expression tag
Asn 324	-	expression tag
Arg 325	-	expression tag
Phe 326	-	expression tag
Lys 327	-	expression tag
Lys 328	-	expression tag
Ile 329	-	expression tag
Ser 330	-	expression tag
Ser 331	-	expression tag
Ser 332	-	expression tag
Gly 333	-	expression tag
Ala 334	-	expression tag
Leu 335	-	expression tag
Asp 336	-	expression tag
Tyr 337	-	expression tag
Asp 338	-	expression tag
Ile 339	-	expression tag
Pro 340	-	expression tag
Thr 341	-	expression tag
Thr 342	-	expression tag
Ala 343	-	expression tag
Ser 344	-	expression tag
Glu 345	-	expression tag
Asn 346	-	expression tag
Leu 347	-	expression tag
Tyr 348	-	expression tag
Phe 349	-	expression tag
Gln 350	-	expression tag

H: 1 - 52 (UniProt: P53207)
H: 1262 - 1314 (PDB: 6N7P)

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	944025.9
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	944222.1

*Water molecules are not included.