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6N61

Escherichia coli RNA polymerase sigma70-holoenzyme bound to upstream fork promoter DNA and Capistruin

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, BDNA-directed RNA polymerase subunit alphapolymer23926459.12UniProt (P0A7Z4)
Pfam (PF01193)
Pfam (PF01000)
In PDB
Escherichia coliRNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2CDNA-directed RNA polymerase subunit betapolymer1342150820.91UniProt (P0A8V2)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
Pfam (PF04560)
In PDB
Escherichia coliRNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3DDNA-directed RNA polymerase subunit beta'polymer1409155577.01UniProt (P0A8T7)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
Pfam (PF05000)
Pfam (PF04998)
In PDB
Escherichia coliRNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4EDNA-directed RNA polymerase subunit omegapolymer9010092.41UniProt (P0A800)
Pfam (PF01192)
In PDB
Escherichia coliRNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5FRNA polymerase sigma factor RpoDpolymer61370351.31UniProt (Q0P6L9)
Pfam (PF03979)
Pfam (PF00140)
Pfam (PF04546)
Pfam (PF04542)
Pfam (PF04539)
Pfam (PF04545)
In PDB
Escherichia coliSigma-70
6Nnon-template strand DNApolymer298924.81Escherichia coli
7Ttemplate strand DNApolymer247363.81Escherichia coli
8ICapistruinpolymer192069.31UniProt (A0A096YQF1)
In PDB
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
9C1,2-ETHANEDIOLnon-polymer62.11Chemie (EDO)
10DMAGNESIUM IONnon-polymer24.31Chemie (MG)
11DZINC IONnon-polymer65.42Chemie (ZN)
12D4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACIDnon-polymer238.31Chemie (EPE)
13waterwater18.026Chemie (HOH)
Sequence modifications
A, B: 1 - 234 (UniProt: P0A7Z4)
PDBExternal DatabaseDetails
Glu 235-expression tag
Val 236-expression tag
Leu 237-expression tag
Phe 238-expression tag
Gln 239-expression tag
D: 2 - 1407 (UniProt: P0A8T7)
PDBExternal DatabaseDetails
Val 1-expression tag
Leu 1408-expression tag
Glu 1409-expression tag
F: 1 - 613 (UniProt: Q0P6L9)
PDBExternal DatabaseDetails
Asn 149Asp 149conflict
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains9
Total formula weight458117.5
Non-Polymers*Number of molecules5
Total formula weight455.5
All*Total formula weight458573.0
*Water molecules are not included.

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PDB entries from 2024-09-18

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