6N57

Cryo-EM structure of Escherichia coli RNAP polymerase bound with TraR in conformation I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	J	DNA-directed RNA polymerase subunit beta'	polymer	1430	158073.6	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	L	RNA polymerase sigma factor RpoD	polymer	616	70715.6	1	UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) In PDB	Escherichia coli	Sigma-70
6	M	Protein TraR	polymer	72	8193.3	1	UniProt (P41065) Pfam (PF01258) In PDB	Escherichia coli	RNA polymerase transcription factor TraR
7	I, J, L, M	CHAPSO	non-polymer		631.9	4	Chemie (1N7)
8	J, M	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
9	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

J: 2 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag
Gly 1416	-	expression tag
Pro 1417	-	expression tag
Ser 1418	-	expression tag
Ser 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

L: 1 - 613 (UniProt: Q0P6L9)

PDB	External Database	Details
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	471170.3
Non-Polymers*	Number of molecules	8
	Total formula weight	2748.1
All*	Total formula weight	473918.4

*Water molecules are not included.