6MYW

Gluconobacter Ene-Reductase (GluER) mutant - T36A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ethylmaleimide reductase	polymer	369	40328.3	4	UniProt (A1E8I9) Pfam (PF00724)	Gluconobacter oxydans	NADH oxidase
2	E, I, O, Z (A, B, C, D)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	4	Chemie (FMN)
3	AA, F, J, P (D, A, B, C)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
4	BA, CA, DA, G, H... (D, A, B, C)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
5	M, N, T, U, V... (B, C)	SODIUM ION	non-polymer		23.0	7	Chemie (NA)
6	Y (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	EA, FA, GA, HA (A, B, C, D)	water	water		18.0	1978	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 361 (UniProt: A1E8I9)

PDB	External Database	Details
Ala 36	Thr 36	engineered mutation
Leu 362	-	expression tag
Glu 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	161313.1
Non-Polymers*	Number of molecules	26
	Total formula weight	3275.2
All*	Total formula weight	164588.3

*Water molecules are not included.