6MUU

Cryo-EM structure of Csm-crRNA binary complex in type III-A CRISPR-Cas system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Uncharacterized protein Csm1	polymer	791	89750.6	1	UniProt (B6YWB8) Pfam (PF01966) Pfam (PF18211) Pfam (PF22335)	Thermococcus onnurineus
2	B (B)	Uncharacterized protein Csm2	polymer	187	21210.3	1	UniProt (B6YWB9) Pfam (PF03750)	Thermococcus onnurineus
3	C, D (C, D)	Uncharacterized protein Csm3	polymer	291	32809.0	2	UniProt (B6YWC0) Pfam (PF03787)	Thermococcus onnurineus
4	E (E)	Uncharacterized protein Csm4	polymer	289	32345.1	1	UniProt (B6YWC1)	Thermococcus onnurineus
5	F (F)	Uncharacterized protein Csm5	polymer	403	46091.0	1	UniProt (B6YWC2) Pfam (PF03787)	Thermococcus onnurineus
6	G (G)	RNA (25-MER)	polymer	38	12463.4	1		Thermococcus onnurineus
7	H, I, J (A, C, D)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)

Sequence modifications

A: 1 - 777 (UniProt: B6YWB8)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

B: 1 - 186 (UniProt: B6YWB9)

PDB	External Database	Details
Ser 0	-	expression tag

C, D: 1 - 290 (UniProt: B6YWC0)

PDB	External Database	Details
Ser 0	-	expression tag

F: 1 - 397 (UniProt: B6YWC2)

PDB	External Database	Details
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	267478.4
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	267674.7

*Water molecules are not included.