6MUT
Cryo-EM structure of ternary Csm-crRNA-target RNA with anti-tag sequence complex in type III-A CRISPR-Cas system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Uncharacterized protein Csm1 | polymer | 791 | 89750.6 | 1 | UniProt (B6YWB8) | Thermococcus onnurineus | |
| 2 | B (B) | Uncharacterized protein Csm2 | polymer | 187 | 21210.3 | 1 | UniProt (B6YWB9) | Thermococcus onnurineus | |
| 3 | C, D (C, D) | Uncharacterized protein Csm3 | polymer | 291 | 32765.0 | 2 | UniProt (B6YWC0) | Thermococcus onnurineus | |
| 4 | E (E) | Uncharacterized protein Csm4 | polymer | 289 | 32345.1 | 1 | UniProt (B6YWC1) | Thermococcus onnurineus | |
| 5 | F (G) | RNA (5'-R(*GP*UP*GP*GP*AP*AP*AP*GP*GP*CP*GP*GP*GP*CP*AP*GP*AP*GP*GP*C)-3') | polymer | 38 | 12463.4 | 1 | Thermococcus onnurineus | ||
| 6 | G (H) | RNA (5'-R(P*CP*CP*UP*CP*UP*GP*CP*CP*CP*GP*CP*CP*UP*UP*UP*CP*CP*AP*C)-3') | polymer | 45 | 14223.5 | 1 | Thermococcus onnurineus | ||
| 7 | H (F) | Uncharacterized protein Csm5 | polymer | 403 | 46091.0 | 1 | UniProt (B6YWC2) | Thermococcus onnurineus | |
| 8 | I, J, K (A, C, D) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A: 1 - 777 (UniProt: B6YWB8)
B: 1 - 186 (UniProt: B6YWB9)
C, D: 1 - 290 (UniProt: B6YWC0)
F: 1 - 397 (UniProt: B6YWC2)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 36 | Asp 36 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 398 | - | expression tag |
| His 399 | - | expression tag |
| His 400 | - | expression tag |
| His 401 | - | expression tag |
| His 402 | - | expression tag |
| His 403 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 281613.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 281810.1 |
