6MSE
Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (U) | 26S proteasome non-ATPase regulatory subunit 1 | polymer | 953 | 105958.2 | 1 | UniProt (Q99460) Pfam (PF21505) Pfam (PF01851) Pfam (PF13646) Pfam (PF18004) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112 |
| 2 | B (V) | 26S proteasome non-ATPase regulatory subunit 3 | polymer | 533 | 60935.3 | 1 | UniProt (O43242) Pfam (PF01399) Pfam (PF08375) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN3,26S proteasome regulatory subunit S3,Proteasome subunit p58 |
| 3 | C (W) | 26S proteasome non-ATPase regulatory subunit 12 | polymer | 456 | 52979.4 | 1 | UniProt (O00232) Pfam (PF22241) Pfam (PF01399) Pfam (PF18098) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN5,26S proteasome regulatory subunit p55 |
| 4 | D (X) | 26S proteasome non-ATPase regulatory subunit 11 | polymer | 422 | 47526.7 | 1 | UniProt (O00231) Pfam (PF18055) Pfam (PF01399) Pfam (PF18503) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN6,26S proteasome regulatory subunit S9,26S proteasome regulatory subunit p44.5 |
| 5 | E (Y) | 26S proteasome non-ATPase regulatory subunit 6 | polymer | 389 | 45592.3 | 1 | UniProt (Q15008) Pfam (PF10602) Pfam (PF01399) Pfam (PF21154) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN7,26S proteasome regulatory subunit S10,Breast cancer-associated protein SGA-113M,Phosphonoformate immuno-associated protein 4,Proteasome regulatory particle subunit p44S10,p42A |
| 6 | F (Z) | 26S proteasome non-ATPase regulatory subunit 7 | polymer | 324 | 37086.4 | 1 | UniProt (P51665) Pfam (PF01398) Pfam (PF13012) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN8,26S proteasome regulatory subunit S12,Mov34 protein homolog,Proteasome subunit p40 |
| 7 | G (a) | 26S proteasome non-ATPase regulatory subunit 13 | polymer | 376 | 42995.4 | 1 | UniProt (Q9UNM6) Pfam (PF22037) Pfam (PF01399) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN9,26S proteasome regulatory subunit S11,26S proteasome regulatory subunit p40.5 |
| 8 | H (b) | 26S proteasome non-ATPase regulatory subunit 4 | polymer | 377 | 40781.6 | 1 | UniProt (P55036) Pfam (PF13519) Pfam (PF02809) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Antisecretory factor 1,ASF,Multiubiquitin chain-binding protein |
| 9 | I (c) | 26S proteasome non-ATPase regulatory subunit 14 | polymer | 309 | 34488.8 | 1 | UniProt (O00487) Pfam (PF01398) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN11,26S proteasome-associated PAD1 homolog 1 |
| 10 | J (d) | 26S proteasome non-ATPase regulatory subunit 8 | polymer | 349 | 39536.7 | 1 | UniProt (P48556) Pfam (PF10075) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN12,26S proteasome regulatory subunit S14,p31 |
| 11 | K (e) | 26S proteasome complex subunit SEM1 | polymer | 70 | 8284.6 | 1 | UniProt (P60896) Pfam (PF05160) | Homo sapiens (Human) | 26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein |
| 12 | L (f) | 26S proteasome non-ATPase regulatory subunit 2 | polymer | 892 | 98425.4 | 1 | UniProt (Q13200) Pfam (PF17781) Pfam (PF01851) Pfam (PF18051) | Homo sapiens (Human) | 26S proteasome regulatory subunit RPN1,26S proteasome regulatory subunit S2,26S proteasome subunit p97,Protein 55.11,Tumor necrosis factor type 1 receptor-associated protein 2 |
| 13 | M (A) | 26S proteasome regulatory subunit 7 | polymer | 433 | 48700.8 | 1 | UniProt (P35998) Pfam (PF21236) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2,Protein MSS1 |
| 14 | N (B) | 26S proteasome regulatory subunit 4 | polymer | 440 | 49260.5 | 1 | UniProt (P62191) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1 |
| 15 | O (C) | 26S proteasome regulatory subunit 8 | polymer | 398 | 44852.1 | 1 | UniProt (P62195) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG |
| 16 | P (D) | 26S proteasome regulatory subunit 6B | polymer | 418 | 47426.1 | 1 | UniProt (P43686) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7 |
| 17 | Q (E) | 26S proteasome regulatory subunit 10B | polymer | 403 | 45867.0 | 1 | UniProt (A0A087X2I1) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) UniProt (by SIFTS) (P62333) | Homo sapiens (Human) | |
| 18 | R (F) | 26S proteasome regulatory subunit 6A | polymer | 439 | 49266.5 | 1 | UniProt (P17980) Pfam (PF16450) Pfam (PF00004) Pfam (PF17862) | Homo sapiens (Human) | 26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1 |
| 19 | S (u) | Ubiquitin | polymer | 76 | 8604.8 | 1 | UniProt (P0CG47) Pfam (PF00240) | Homo sapiens (Human) | |
| 20 | T (v) | substrate | polymer | 28 | 2445.0 | 1 | Saccharomyces cerevisiae RM11-1a (Yeast) | ||
| 21 | IA, U (G, g) | Proteasome subunit alpha type-6 | polymer | 245 | 27301.3 | 2 | UniProt (P60900) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain |
| 22 | JA, V (H, h) | Proteasome subunit alpha type-2 | polymer | 233 | 25796.3 | 2 | UniProt (P25787) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3 |
| 23 | KA, W (I, i) | Proteasome subunit alpha type-4 | polymer | 260 | 29394.6 | 2 | UniProt (P25789) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L |
| 24 | LA, X (J, j) | Proteasome subunit alpha type-7 | polymer | 247 | 27798.7 | 2 | UniProt (O14818) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Proteasome subunit RC6-1,Proteasome subunit XAPC7 |
| 25 | MA, Y (K, k) | Proteasome subunit alpha type-5 | polymer | 240 | 26304.8 | 2 | UniProt (P28066) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome zeta chain |
| 26 | NA, Z (L, l) | Proteasome subunit alpha type-1 | polymer | 268 | 30150.3 | 2 | UniProt (P25786) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | 30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain |
| 27 | AA, OA (M, m) | Proteasome subunit alpha type-3 | polymer | 254 | 28338.1 | 2 | UniProt (P25788) Pfam (PF10584) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8 |
| 28 | BA, PA (N, n) | Proteasome subunit beta type-6 | polymer | 238 | 25246.5 | 2 | UniProt (P28072) Pfam (PF00227) | Homo sapiens (Human) | Macropain delta chain,Multicatalytic endopeptidase complex delta chain,Proteasome delta chain,Proteasome subunit Y |
| 29 | CA, QA (O, o) | Proteasome subunit beta type-7 | polymer | 276 | 29869.2 | 2 | UniProt (Q99436) Pfam (PF00227) Pfam (PF12465) | Homo sapiens (Human) | Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z |
| 30 | DA, RA (P, p) | Proteasome subunit beta type-3 | polymer | 204 | 22841.7 | 2 | UniProt (P49720) Pfam (PF00227) | Homo sapiens (Human) | Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain |
| 31 | EA, SA (Q, q) | Proteasome subunit beta type-2 | polymer | 201 | 22864.3 | 2 | UniProt (P49721) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C7-I,Multicatalytic endopeptidase complex subunit C7-I,Proteasome component C7-I |
| 32 | FA, TA (R, r) | Proteasome subunit beta type-5 | polymer | 262 | 28379.1 | 2 | UniProt (P28074) Pfam (PF00227) | Homo sapiens (Human) | Macropain epsilon chain,Multicatalytic endopeptidase complex epsilon chain,Proteasome chain 6,Proteasome epsilon chain,Proteasome subunit MB1,Proteasome subunit X |
| 33 | GA, UA (S, s) | Proteasome subunit beta type-1 | polymer | 240 | 26391.2 | 2 | UniProt (P20618) Pfam (PF00227) | Homo sapiens (Human) | Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain |
| 34 | HA, VA (T, t) | Proteasome subunit beta type-4 | polymer | 263 | 29100.0 | 2 | UniProt (P28070) Pfam (PF00227) | Homo sapiens (Human) | 26 kDa prosomal protein,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3 |
| 35 | WA (c) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 36 | AB, DB, XA (D, F, A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 3 | Chemie (ATP) | |||
| 37 | BB, EB, YA (D, F, A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 38 | CB, ZA (E, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) |
Sequence modifications
u: 1 - 76 (UniProt: P0CG47)
| PDB | External Database | Details |
|---|---|---|
| Arg 63 | Lys 63 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 48 |
| Total formula weight | 1670565.6 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2514.3 | |
| All* | Total formula weight | 1673079.9 |
