6MP7

Crystal structure of the E257A mutant of BlMan5B in complex with GlcNAc (soaking)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	BlMan5B	polymer	449	49605.6	2	UniProt (B3DQP5) In PDB	Bifidobacterium longum (strain DJO10A)
2	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
3	A	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
4	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5		water	water		18.0	589	Chemie (HOH)

Sequence modifications

A, B: 1 - 429 (UniProt: B3DQP5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 257	Glu 257	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99211.1
Non-Polymers*	Number of molecules	4
	Total formula weight	654.5
All*	Total formula weight	99865.7

*Water molecules are not included.