6MI7

Nucleotide-free Cryo-EM Structure of E.coli LptB2FGC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (F)	Lipopolysaccharide export system permease protein LptF	polymer	366	40393.5	1	UniProt (P0AF98) Pfam (PF03739)	Escherichia coli (strain K12)
2	B (G)	Lipopolysaccharide export system permease protein LptG	polymer	360	39651.4	1	UniProt (P0ADC6) Pfam (PF03739)	Escherichia coli (strain K12)
3	C, D (A, B)	Lipopolysaccharide export system ATP-binding protein LptB	polymer	251	28131.1	2	UniProt (P0A9V1) Pfam (PF00005) Pfam (PF12399)	Escherichia coli (strain K12)
4	E (C)	Lipopolysaccharide export system protein LptC	polymer	208	23808.9	1	UniProt (P0ADV9) Pfam (PF06835)	Escherichia coli (strain K12)
5	F (F)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	1	Chemie (PGT)

Sequence modifications

A, B: 1 - 241 (UniProt: P0A9V1)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C: 1 - 191 (UniProt: P0ADV9)

PDB	External Database	Details
Met -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	160115.9
Non-Polymers*	Number of molecules	1
	Total formula weight	751.0
All*	Total formula weight	160867.0

*Water molecules are not included.