6MHU

Nucleotide-free Cryo-EM Structure of E.coli LptB2FG Transporter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (F)	Lipopolysaccharide export system permease protein LptF	polymer	366	40393.5	1	UniProt (P0AF98) Pfam (PF03739)	Escherichia coli (strain K12)
2	B (G)	Lipopolysaccharide export system permease protein LptG	polymer	360	39651.4	1	UniProt (P0ADC6) Pfam (PF03739)	Escherichia coli (strain K12)
3	C, D (A, B)	Lipopolysaccharide export system ATP-binding protein LptB	polymer	251	28131.1	2	UniProt (P0A9V1) Pfam (PF00005) Pfam (PF12399)	Escherichia coli (strain K12)
4	E (G)	(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-5-[(2~{S},3~{S},4~{R},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{R},5~{S},6~{R})-6-[(1~{S})-2-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-1-oxidanyl-ethyl]-3,4-bis(oxidanyl)-5-phosphonooxy-oxan-2-yl]oxy-3-oxidanyl-5-phosphonooxy-oxan-2-yl]oxy-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid	non-polymer		2975.2	1	Chemie (JSG)

Sequence modifications

A, B: 1 - 241 (UniProt: P0A9V1)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136307.1
Non-Polymers*	Number of molecules	1
	Total formula weight	2975.2
All*	Total formula weight	139282.2

*Water molecules are not included.