6MDM

The 20S supercomplex engaging the SNAP-25 N-terminus (class 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Vesicle-fusing ATPase	polymer	768	85509.2	6	UniProt (P18708) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) Pfam (PF21964)	Cricetulus griseus (Chinese hamster)	N-ethylmaleimide-sensitive fusion protein,NEM-sensitive fusion protein,Vesicular-fusion protein NSF
2	G (H)	Synaptosomal-associated protein 25	polymer	207	23512.4	1	UniProt (P60881) Pfam (PF00835)	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
3	H (I)	Syntaxin-1A	polymer	256	29634.1	1	UniProt (P32851) Pfam (PF00804) Pfam (PF05739)	Rattus norvegicus (Rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
4	I (J)	Vesicle-associated membrane protein 2	polymer	117	12981.3	1	UniProt (P63045) Pfam (PF00957)	Rattus norvegicus (Rat)	VAMP-2,Synaptobrevin-2
5	J, K (K, L)	Alpha-soluble NSF attachment protein	polymer	313	35598.2	2	UniProt (P54921)	Rattus norvegicus (Rat)	SNAP-alpha,N-ethylmaleimide-sensitive factor attachment protein alpha
6	L, N, O, P, Q... (A, B, C, D, E...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	9	Chemie (ATP)
7	M, U (A, E)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, B, C, D, E, F: 1 - 744 (UniProt: P18708)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag
Ile 458	Lys 458	conflict

H: 1 - 204 (UniProt: P60881)

PDB	External Database	Details
Met -2	-	initiating methionine
Ala -1	-	expression tag
Ser 0	-	expression tag

I: 1 - 256 (UniProt: P32851)

PDB	External Database	Details
Ser 145	Cys 145	conflict

J: 1 - 89 (UniProt: P63045)

PDB	External Database	Details
Met -27	-	initiating methionine
Ala -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
Tyr -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Tyr -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Pro -12	-	expression tag
Thr -11	-	expression tag
Ser -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

K, L: 1 - 295 (UniProt: P54921)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	650379.6
Non-Polymers*	Number of molecules	11
	Total formula weight	5419.0
All*	Total formula weight	655798.7

*Water molecules are not included.