6M83

Crystal structure of TylM1 S120A bound to SAH and dTDP-phenol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase	polymer	263	28521.9	1	UniProt (P95748) Pfam (PF13649) In PDB	Streptomyces fradiae	Tylosin biosynthesis protein M1
2	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
3	A	5'-O-[(S)-hydroxy{[(S)-hydroxy(phenoxy)phosphoryl]oxy}phosphoryl]thymidine	non-polymer		478.3	1	Chemie (TLO)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	217	Chemie (HOH)

Sequence modifications

A: 1 - 255 (UniProt: P95748)

PDB	External Database	Details
Ala 120	Ser 120	engineered mutation
Leu 256	-	expression tag
Glu 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28521.9
Non-Polymers*	Number of molecules	3
	Total formula weight	898.1
All*	Total formula weight	29420.0

*Water molecules are not included.