6M81

Crystal structure of TylM1 Y14F bound to SAH and dTDP-phenol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase	polymer	263	28521.9	4	UniProt (P95748) Pfam (PF13649)	Streptomyces fradiae	Tylosin biosynthesis protein M1
2	E, G, I, K (A, B, C, D)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	4	Chemie (SAH)
3	F, H, J, L (A, B, C, D)	5'-O-[(S)-hydroxy{[(S)-hydroxy(phenoxy)phosphoryl]oxy}phosphoryl]thymidine	non-polymer		478.3	4	Chemie (TLO)
4	M, N, O, P (A, B, C, D)	water	water		18.0	785	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 255 (UniProt: P95748)

PDB	External Database	Details
Phe 14	Tyr 14	engineered mutation
Leu 256	-	expression tag
Glu 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	114087.5
Non-Polymers*	Number of molecules	8
	Total formula weight	3450.8
All*	Total formula weight	117538.3

*Water molecules are not included.