6M7Y

Dehydratase, NisB, bound to a non-eliminable substrate analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Nisin biosynthesis protein NisB	polymer	996	118647.1	2	UniProt (P20103)	Lactococcus lactis subsp. lactis
2	C, D (C, D)	Lantibiotic	polymer	34	3641.1	2	UniProt (Q7BB86) UniProt (by SIFTS) (P29559)	Lactococcus lactis
3	E, F, G, H (A, B, C, D)	water	water		18.0	247	Chemie (HOH)

Sequence modifications

A, B: 2 - 993 (UniProt: P20103)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Mse 1	-	expression tag
Cys 169	Val 169	engineered mutation

C, D: -22 - 12 (UniProt: Q7BB86)

PDB	External Database	Details
Cys -12	Ser 12	engineered mutation
J9a 3	Ser 26	engineered mutation
Ala 7	Cys 30	engineered mutation
Ala 11	Cys 34	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	244576.5
All*	Total formula weight	244576.5

*Water molecules are not included.