6M63

Crystal structure of a cAMP sensor G-Flamp1.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Chimera of Cyclic nucleotide-gated potassium channel mll3241 and Yellow fluorescent protein	polymer	389	42620.6	2	UniProt (Q98GN8) UniProt (A0A059PIR9) Pfam (PF00027) Pfam (PF01353) UniProt (by SIFTS) (P42212)	Mesorhizobium japonicum MAFF 303099	MlotiK1 channel
2	C, D (A, B)	ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE	non-polymer		329.2	2	Chemie (CMP)
3	E, F (A, B)	water	water		18.0	365	Chemie (HOH)

Sequence modifications

A, B: 2 - 73 (UniProt: Q98GN8)

PDB	External Database	Details
Met 1	-	initiating methionine

A, B: 77 - 166 (UniProt: A0A059PIR9)

PDB	External Database	Details
Gly 101	Asp 173	engineered mutation
Gly 103	Ser 175	engineered mutation
Tyr 108	Asp 180	engineered mutation
Val 131	Tyr 203	engineered mutation
Lys 134	Ala 206	engineered mutation
Leu 159	His 231	engineered mutation

A, B: 176 - 317 (UniProt: A0A059PIR9)

PDB	External Database	Details
Gly 167	-	linker
Gly 168	-	linker
Thr 169	-	linker
Gly 170	-	linker
Gly 171	-	linker
Ser 172	-	linker
Met 173	-	linker
Val 174	-	linker
Ser 175	-	linker
Arg 203	Ser 30	engineered mutation
Cro 239	Thr 65	chromophore
Cro 239	Tyr 66	chromophore
Cro 239	Gly 67	chromophore
Ile 266	Val 93	engineered mutation
Val 272	Ser 99	engineered mutation
Arg 318	-	linker
Val 319	-	linker

A, B: 320 - 389 (UniProt: Q98GN8)

PDB	External Database	Details
Asn 74	-	linker
Trp 75	-	linker
Gly 76	-	linker
Val 342	Ser 308	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85241.2
Non-Polymers*	Number of molecules	2
	Total formula weight	658.4
All*	Total formula weight	85899.6

*Water molecules are not included.