6M4N

Cryo-EM structure of the dimeric SPT-ORMDL3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Serine palmitoyltransferase 1	polymer	473	52806.8	2	UniProt (O15269) Pfam (PF00155)	Homo sapiens (Human)	Long chain base biosynthesis protein 1,LCB 1,Serine-palmitoyl-CoA transferase 1,SPT1
2	B, F (B, F)	Serine palmitoyltransferase 2	polymer	562	63004.2	2	UniProt (O15270) Pfam (PF00155)	Homo sapiens (Human)	Long chain base biosynthesis protein 2,LCB 2,Long chain base biosynthesis protein 2a,LCB2a,Serine-palmitoyl-CoA transferase 2,SPT 2
3	C, G (C, G)	ORM1-like protein 3	polymer	153	17512.6	2	UniProt (Q8N138) Pfam (PF04061)	Homo sapiens (Human)
4	D, H (D, H)	Serine palmitoyltransferase small subunit A	polymer	92	10742.4	2	UniProt (Q969W0) Pfam (PF11779)	Homo sapiens (Human)	Small subunit of serine palmitoyltransferase A,ssSPTa
5	I, J (B, F)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)

Sequence modifications

D, H: 1 - 71 (UniProt: Q969W0)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	288131.9
Non-Polymers*	Number of molecules	2
	Total formula weight	494.3
All*	Total formula weight	288626.2

*Water molecules are not included.