6M4M
X-ray crystal structure of the E249Q mutan of alpha-amylase I and maltohexaose complex from Eisenia fetida
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alpha-amylase | polymer | 520 | 58348.9 | 1 | UniProt (A0A173N065) Pfam (PF00128) Pfam (PF02806) In PDB | Eisenia fetida (Red wiggler worm) | |
| 2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 990.9 | 1 | In PDB BIRD (PRD_900035) GlyTouCan (G91368RN) | alpha-maltohexaose | ||
| 3 | C | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
| 4 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | A | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 7 | A | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 8 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 9 | water | water | 18.0 | 511 | Chemie (HOH) |
Sequence modifications
A: 18 - 510 (UniProt: A0A173N065)
| PDB | External Database | Details |
|---|---|---|
| Glu 14 | - | expression tag |
| Ala 15 | - | expression tag |
| Glu 16 | - | expression tag |
| Phe 17 | - | expression tag |
| Gln 249 | Glu 249 | engineered mutation |
| Phe 511 | - | expression tag |
| Leu 512 | - | expression tag |
| Glu 513 | - | expression tag |
| Gln 514 | - | expression tag |
| Lys 515 | - | expression tag |
| Leu 516 | - | expression tag |
| Ile 517 | - | expression tag |
| Ser 518 | - | expression tag |
| Glu 519 | - | expression tag |
| Glu 520 | - | expression tag |
| Asp 521 | - | expression tag |
| Leu 522 | - | expression tag |
| Asn 523 | - | expression tag |
| Ser 524 | - | expression tag |
| Ala 525 | - | expression tag |
| Val 526 | - | expression tag |
| Asp 527 | - | expression tag |
| His 528 | - | expression tag |
| His 529 | - | expression tag |
| His 530 | - | expression tag |
| His 531 | - | expression tag |
| His 532 | - | expression tag |
| His 533 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 58348.9 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1657.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 996.3 | |
| All* | Total formula weight | 61002.7 |
