6M4L

X-ray crystal structure of the E249Q mutant of alpha-amylase I from Eisenia fetida

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha-amylase	polymer	520	58348.9	1	UniProt (A0A173N065) Pfam (PF00128) Pfam (PF02806) In PDB	Eisenia fetida (Red wiggler worm)
2	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	A	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
7	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
8	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
9	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
10		water	water		18.0	515	Chemie (HOH)

Sequence modifications

A: 18 - 510 (UniProt: A0A173N065)

PDB	External Database	Details
Glu 14	-	expression tag
Ala 15	-	expression tag
Glu 16	-	expression tag
Phe 17	-	expression tag
Gln 249	Glu 249	engineered mutation
Phe 511	-	expression tag
Leu 512	-	expression tag
Glu 513	-	expression tag
Gln 514	-	expression tag
Lys 515	-	expression tag
Leu 516	-	expression tag
Ile 517	-	expression tag
Ser 518	-	expression tag
Glu 519	-	expression tag
Glu 520	-	expression tag
Asp 521	-	expression tag
Leu 522	-	expression tag
Asn 523	-	expression tag
Ser 524	-	expression tag
Ala 525	-	expression tag
Val 526	-	expression tag
Asp 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag
His 531	-	expression tag
His 532	-	expression tag
His 533	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58348.9
Non-Polymers*	Number of molecules	14
	Total formula weight	1328.8
All*	Total formula weight	59677.7

*Water molecules are not included.