6M4F

Crystal structure of the E496A mutant of HsBglA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, C, E, G)	Beta-galactosidase-like enzyme	polymer	578	64288.2	4	UniProt (Q564N5) Pfam (PF00232)	Hamamotoa singularis
2	E, F, G, H, I... (B, D, F, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
3	M, N, R, T, U... (A, C, E, G)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	8	Chemie (NAG)
4	O, P, Q, S, V (C, E, G)	alpha-D-mannopyranose	non-polymer		180.2	5	Chemie (MAN)
5	AA, BA, CA, Z (C, E, G, A)	water	water		18.0	1060	Chemie (HOH)

Sequence modifications

A, C, E, G: 23 - 594 (UniProt: Q564N5)

PDB	External Database	Details
Ala 496	Glu 496	engineered mutation
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag
His 598	-	expression tag
His 599	-	expression tag
His 600	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	257152.8
Branched	Number of molecules	8
	Total formula weight	3395.2
Non-Polymers*	Number of molecules	13
	Total formula weight	2670.4
All*	Total formula weight	263218.4

*Water molecules are not included.