6M49

cryo-EM structure of Scap/Insig complex in the present of 25-hydroxyl cholesterol.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Insulin-induced gene 2 protein	polymer	225	24749.7	1	UniProt (Q9Y5U4) Pfam (PF07281) In PDB	Homo sapiens (Human)	INSIG-2
2	B	Sterol regulatory element-binding protein cleavage-activating protein,Sterol regulatory element-binding protein cleavage-activating protein	polymer	572	63295.4	1	UniProt (Q12770) Pfam (PF12349) In PDB	Homo sapiens (Human)	SREBP cleavage-activating protein,SREBP cleavage-activating protein
3	A	25-HYDROXYCHOLESTEROL	non-polymer		402.7	1	Chemie (HC3)

Sequence modifications

A: 1 - 225 (UniProt: Q9Y5U4)

PDB	External Database	Details
Ser 14	Cys 14	engineered mutation
Ser 90	Cys 90	engineered mutation
Ser 215	Cys 215	engineered mutation

B: 1 - 215 (UniProt: Q12770)
B: 216 - 756 (UniProt: Q12770)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88045.1
Non-Polymers*	Number of molecules	1
	Total formula weight	402.7
All*	Total formula weight	88447.7

*Water molecules are not included.