6LTC

Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)-alpha-methyl-L-serine-AMP bound form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nonribosomal peptide synthetase	polymer	1153	125243.7	1	UniProt (A0A077JG85)	Streptomyces sp. Sp080513GE-23
2	B (A)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
3	C (A)	alpha-methyl-L-serine	non-polymer		119.1	1	Chemie (EW6)
4	D, E, F, G (A)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	H, I, J, K, L... (A)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
6	N (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 1127 (UniProt: A0A077JG85)

PDB	External Database	Details
Met -15	-	expression tag
Asn -14	-	expression tag
His -13	-	expression tag
Lys -12	-	expression tag
Val -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ile -4	-	expression tag
Glu -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
His 0	-	expression tag
Ala 1046	Ser 1046	engineered mutation
Ser 1128	-	expression tag
Ala 1129	-	expression tag
Trp 1130	-	expression tag
Ser 1131	-	expression tag
His 1132	-	expression tag
Pro 1133	-	expression tag
Gln 1134	-	expression tag
Phe 1135	-	expression tag
Glu 1136	-	expression tag
Lys 1137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	125243.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1063.3
All*	Total formula weight	126307.0

*Water molecules are not included.